| 1. | In order to overcome this deficiency, the concept of activity or effective concentration was advanced .
为了克服这一缺点，提出了活度或有效浓度的概念。 |
| 2. | 2 , the effective bactericidal concentration is lower . the effective concentration of common iodophor is 50 - 100ppm
2 、有效杀菌浓度更低：一般碘伏有效浓度为50100ppm 。 |
| 3. | Quantitative investigations have shown that mps penetrates into deeper tissue layers and that therapeutic effective concentrations are achieved
定量的观察还显示mps (多磺酸粘多糖）不但能渗透入更深的组织层而且能达到有效的治疗浓度。 |
| 4. | Effective concentration of cef restrain agrobacterium tumefaciens is 200 | xg / ml . the efficient density of as is 100 umol / l in the co - cultivatio
5表面活性剂tween20 ，可以使gus染色均匀，但并不是t dna转移所必需，盐的浓度对t dna转移影响不大。 |
| 5. | Gibberillin of 50 - 300mg / l promoted flowering of young seedlings and adult plants in cv . youdonger and the most effective concentration was 300 mg / l
50 - 300mg l的赤霉素处理都可以促进油冬儿白菜开花，无论大苗和小苗均有效果，以300mg l为最佳处理浓度。 |
| 6. | The results not only confirmed the absorption of mps but also showed that the active ingredient reaches the individual dermal layers in therapeutic effective concentrations
研究结论证实mps （多磺酸粘多糖）不但能透皮吸收，而且其活性成分能在各真皮亚层达到有效的治疗浓度。 |
| 7. | Bth had a higher efficiency to induce gus expression than sa indicated by gus staining strength and had no phytotoxicity to plant within effective concentration . 5
化学诱导剂bth的诱导效率高于sa ，且在有效诱导浓度范围内对植株没有生理毒性或所产生的生理毒性难以从表象观察到。 |
| 8. | The highest non - effective concentration ( noec ) and the lowest effective concentration ( loec ) , as counted with number of youth per female , covered the range of 0 . 005 - 0 . 01 g / l
根据产蚤数指标，氰戊菊酯和高效氯氰菊酯对大型蚤的最高无影响浓度( noec )以及最低有影响浓度( loec )分别介于0 . 005 0 . 01 g l之间。 |
| 9. | Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan , and be stimulated by ifn - r before oligochitosan added , then measured the changes of gene transcription and translation level of both il - 1 and imf - a , respectively by methods of relatively quantitive rt - pcr and elisa . first , rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan , then by the same method , confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating . because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone , so add ifn - r to microphages alone for 22 hours , then examined by rt - pcr , the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group
此外，由于ifn y单独作用也可促进两种细胞因子基因表达，故在巨噬细胞中加入ifn y单独作用22h ，再经阿一pcr检测，发现加ifn y的实验组细胞的几一lp和tnf a基因转录水平与空白对照组相比较无显著性差异，可见，壳寡糖和ifn v对巨噬细胞il lp和tnf一口基因转录水平的影响在作用时间上无一致性，在壳寡糖作用最适时间时，仅受ifn y刺激的巨噬细胞il lp和tnf q基因转录己下降至刺激前水平，因此可以认为， ifn y的加入仅起到对巨噬细胞预刺激使之处于敏感状态的作用，有利于增强壳寡糖对巨噬细胞的作用。 |
| 10. | This means that joining of ifn - yonly to stimulate and make the macrophages in sensitive state , help to strengthen the enhancing effect of oligochitosan on il - 1 and tnf - a gene expression of macrophages . after confirming the most effective time and the most effective concentration of oligochitosan and ifn - y , select three groups of cells , experiment group join loou / ml ifn - r , after 4 hours , add 40ug / ml oligochitosan ; oligochitosan contrast group add oligochitosan only ; blank contrast groupadd neither ifn - y nor oligochitosan , then the three goups culture 18 hours together
在确定了壳寡糖和ifn y的最适作用时间及最适作用浓度之后，选取三组细胞状态良好，数目比较一致的巨噬细胞，实验组中加入100u mlifn v预刺激4h后，再加入40ug ml壳寡糖，加糖对照组则只加40ng砌l壳寡糖，空白对照组两者都不加，继续培养18h 。 |
